Expressed sequence tags from poplar wood tissues--a comparative analysis from multiple libraries.
Identifieur interne : 004145 ( Main/Exploration ); précédent : 004144; suivant : 004146Expressed sequence tags from poplar wood tissues--a comparative analysis from multiple libraries.
Auteurs : A. Déjardin [France] ; J-C Leplé ; M-C Lesage-Descauses ; G. Costa ; G. PilateSource :
- Plant biology (Stuttgart, Germany) [ 1435-8603 ]
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ADN complémentaire (isolement et purification), ADN des plantes (génétique), ADN des plantes (isolement et purification), ARN des plantes (génétique), ARN des plantes (isolement et purification), Analyse de regroupements (MeSH), Banque de gènes (MeSH), Bois (MeSH), Cycle cellulaire (génétique), Gènes de plante (MeSH), Génome végétal (MeSH), Lignine (métabolisme), Mucoprotéines (génétique), Méthylation (MeSH), Populus (croissance et développement), Populus (génétique), Populus (métabolisme), Protéines végétales (biosynthèse), Protéines végétales (génétique), Étiquettes de séquences exprimées (MeSH).
- MESH :
- biosynthèse : Protéines végétales.
- croissance et développement : Populus.
- génétique : ADN complémentaire, ADN des plantes, ARN des plantes, Cycle cellulaire, Mucoprotéines, Populus, Protéines végétales.
- isolement et purification : ADN complémentaire, ADN des plantes, ARN des plantes.
- métabolisme : Lignine, Populus.
- Analyse de regroupements, Banque de gènes, Bois, Gènes de plante, Génome végétal, Méthylation, Étiquettes de séquences exprimées.
English descriptors
- KwdEn :
- Cell Cycle (genetics), Cluster Analysis (MeSH), DNA, Complementary (genetics), DNA, Complementary (isolation & purification), DNA, Plant (genetics), DNA, Plant (isolation & purification), Expressed Sequence Tags (MeSH), Gene Library (MeSH), Genes, Plant (MeSH), Genome, Plant (MeSH), Lignin (metabolism), Methylation (MeSH), Mucoproteins (genetics), Plant Proteins (biosynthesis), Plant Proteins (genetics), Populus (genetics), Populus (growth & development), Populus (metabolism), RNA, Plant (genetics), RNA, Plant (isolation & purification), Wood (MeSH).
- MESH :
- chemical , biosynthesis : Plant Proteins.
- chemical , genetics : DNA, Complementary, DNA, Plant, Mucoproteins, Plant Proteins, RNA, Plant.
- genetics : Cell Cycle, Populus.
- growth & development : Populus.
- chemical , isolation & purification : DNA, Complementary, DNA, Plant, RNA, Plant.
- chemical , metabolism : Lignin, Populus.
- Cluster Analysis, Expressed Sequence Tags, Gene Library, Genes, Plant, Genome, Plant, Methylation, Wood.
Abstract
Xylogenesis involves successive developmental processes--cambial division, cell expansion and differentiation, cell death--each occurring along a gradient from the cambium to the pith of the stem. Taking advantage of the high level of organisation of wood tissues, we isolated cambial zone (CZ), differentiating xylem (DX) and mature xylem (MX) from both tension wood (TW) and opposite wood (OW) of bent poplars. Four different cDNA libraries were then constructed and used to generate 10,062 EST, reflecting the genes expressed in the different wood tissues. For the most abundant clusters, the EST distributions were compared between libraries in order to identify genes specific or over-represented at some specific developmental stages. They clearly showed a developmental shift between CZ and DX, whereas there is a continuity of development between DX and MX. CZ was mainly characterized by clusters of genes involved in cell cycle, protein synthesis and fate. Interestingly, two clusters with no assigned function were found specific to the cambial zone. In DX and MX, clusters were mostly involved in methylation of lignin precursors and microtubule cytoskeleton. In addition, in DX, EST from TW and OW were compared: five clusters of arabinogalactan proteins, one for sucrose synthase and one for fructokinase were specific or over-represented in TW. Moreover, a putative transcription factor and a cluster of unknown function were also identified in DX-TW. The informative comparison of multiple libraries prepared from wood tissues led to the identification of genes--some with still unknown functions--putatively involved in xylogenesis and tension wood formation.
DOI: 10.1055/s-2003-44744
PubMed: 15095135
Affiliations:
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Déjardin</name><affiliation wicri:level="3"><nlm:affiliation>Unité Amélioration, Génétique et Physiologie Forestières, INRA Orléans, BP 20619 Ardon, 45166 Olivet Cédex, France. dejardin@orleans.inra.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Unité Amélioration, Génétique et Physiologie Forestières, INRA Orléans, BP 20619 Ardon, 45166 Olivet Cédex</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Olivet Cédex</settlement></placeName></affiliation></author><author><name sortKey="Leple, J C" sort="Leple, J C" uniqKey="Leple J" first="J-C" last="Leplé">J-C Leplé</name></author><author><name sortKey="Lesage Descauses, M C" sort="Lesage Descauses, M C" uniqKey="Lesage Descauses M" first="M-C" last="Lesage-Descauses">M-C Lesage-Descauses</name></author><author><name sortKey="Costa, G" sort="Costa, G" uniqKey="Costa G" first="G" last="Costa">G. 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Déjardin</name><affiliation wicri:level="3"><nlm:affiliation>Unité Amélioration, Génétique et Physiologie Forestières, INRA Orléans, BP 20619 Ardon, 45166 Olivet Cédex, France. dejardin@orleans.inra.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Unité Amélioration, Génétique et Physiologie Forestières, INRA Orléans, BP 20619 Ardon, 45166 Olivet Cédex</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Olivet Cédex</settlement></placeName></affiliation></author><author><name sortKey="Leple, J C" sort="Leple, J C" uniqKey="Leple J" first="J-C" last="Leplé">J-C Leplé</name></author><author><name sortKey="Lesage Descauses, M C" sort="Lesage Descauses, M C" uniqKey="Lesage Descauses M" first="M-C" last="Lesage-Descauses">M-C Lesage-Descauses</name></author><author><name sortKey="Costa, G" sort="Costa, G" uniqKey="Costa G" first="G" last="Costa">G. 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Taking advantage of the high level of organisation of wood tissues, we isolated cambial zone (CZ), differentiating xylem (DX) and mature xylem (MX) from both tension wood (TW) and opposite wood (OW) of bent poplars. Four different cDNA libraries were then constructed and used to generate 10,062 EST, reflecting the genes expressed in the different wood tissues. For the most abundant clusters, the EST distributions were compared between libraries in order to identify genes specific or over-represented at some specific developmental stages. They clearly showed a developmental shift between CZ and DX, whereas there is a continuity of development between DX and MX. CZ was mainly characterized by clusters of genes involved in cell cycle, protein synthesis and fate. Interestingly, two clusters with no assigned function were found specific to the cambial zone. In DX and MX, clusters were mostly involved in methylation of lignin precursors and microtubule cytoskeleton. In addition, in DX, EST from TW and OW were compared: five clusters of arabinogalactan proteins, one for sucrose synthase and one for fructokinase were specific or over-represented in TW. Moreover, a putative transcription factor and a cluster of unknown function were also identified in DX-TW. The informative comparison of multiple libraries prepared from wood tissues led to the identification of genes--some with still unknown functions--putatively involved in xylogenesis and tension wood formation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15095135</PMID><DateCompleted><Year>2004</Year><Month>05</Month><Day>19</Day></DateCompleted><DateRevised><Year>2008</Year><Month>01</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1435-8603</ISSN><JournalIssue CitedMedium="Print"><Volume>6</Volume><Issue>1</Issue><PubDate><MedlineDate>2004 Jan-Feb</MedlineDate></PubDate></JournalIssue><Title>Plant biology (Stuttgart, Germany)</Title><ISOAbbreviation>Plant Biol (Stuttg)</ISOAbbreviation></Journal><ArticleTitle>Expressed sequence tags from poplar wood tissues--a comparative analysis from multiple libraries.</ArticleTitle><Pagination><MedlinePgn>55-64</MedlinePgn></Pagination><Abstract><AbstractText>Xylogenesis involves successive developmental processes--cambial division, cell expansion and differentiation, cell death--each occurring along a gradient from the cambium to the pith of the stem. Taking advantage of the high level of organisation of wood tissues, we isolated cambial zone (CZ), differentiating xylem (DX) and mature xylem (MX) from both tension wood (TW) and opposite wood (OW) of bent poplars. Four different cDNA libraries were then constructed and used to generate 10,062 EST, reflecting the genes expressed in the different wood tissues. For the most abundant clusters, the EST distributions were compared between libraries in order to identify genes specific or over-represented at some specific developmental stages. They clearly showed a developmental shift between CZ and DX, whereas there is a continuity of development between DX and MX. CZ was mainly characterized by clusters of genes involved in cell cycle, protein synthesis and fate. Interestingly, two clusters with no assigned function were found specific to the cambial zone. In DX and MX, clusters were mostly involved in methylation of lignin precursors and microtubule cytoskeleton. In addition, in DX, EST from TW and OW were compared: five clusters of arabinogalactan proteins, one for sucrose synthase and one for fructokinase were specific or over-represented in TW. Moreover, a putative transcription factor and a cluster of unknown function were also identified in DX-TW. The informative comparison of multiple libraries prepared from wood tissues led to the identification of genes--some with still unknown functions--putatively involved in xylogenesis and tension wood formation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Déjardin</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Unité Amélioration, Génétique et Physiologie Forestières, INRA Orléans, BP 20619 Ardon, 45166 Olivet Cédex, France. dejardin@orleans.inra.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leplé</LastName><ForeName>J-C</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Lesage-Descauses</LastName><ForeName>M-C</ForeName><Initials>MC</Initials></Author><Author ValidYN="Y"><LastName>Costa</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Pilate</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biol (Stuttg)</MedlineTA><NlmUniqueID>101148926</NlmUniqueID><ISSNLinking>1435-8603</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009088">Mucoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C006619">arabinogalactan proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002453" MajorTopicYN="N">Cell Cycle</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016000" MajorTopicYN="N">Cluster Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020224" MajorTopicYN="N">Expressed Sequence Tags</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018745" MajorTopicYN="N">Genome, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008745" MajorTopicYN="N">Methylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009088" MajorTopicYN="N">Mucoproteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>4</Month><Day>20</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>5</Month><Day>20</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>4</Month><Day>20</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15095135</ArticleId><ArticleId IdType="doi">10.1055/s-2003-44744</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Centre-Val de Loire</li><li>Région Centre</li></region><settlement><li>Olivet Cédex</li></settlement></list><tree><noCountry><name sortKey="Costa, G" sort="Costa, G" uniqKey="Costa G" first="G" last="Costa">G. Costa</name><name sortKey="Leple, J C" sort="Leple, J C" uniqKey="Leple J" first="J-C" last="Leplé">J-C Leplé</name><name sortKey="Lesage Descauses, M C" sort="Lesage Descauses, M C" uniqKey="Lesage Descauses M" first="M-C" last="Lesage-Descauses">M-C Lesage-Descauses</name><name sortKey="Pilate, G" sort="Pilate, G" uniqKey="Pilate G" first="G" last="Pilate">G. Pilate</name></noCountry><country name="France"><region name="Centre-Val de Loire"><name sortKey="Dejardin, A" sort="Dejardin, A" uniqKey="Dejardin A" first="A" last="Déjardin">A. Déjardin</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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